Worldwide, food-borne diseases are a major health burden leading to high morbidity and mortality. Our research focused on safe food handling knowledge and behaviors among industrial kitchen handlers. We described one research mentioned to the description of handling knowledge, behavior about food safety awareness of industrial kitchen handlers (IKHs). Our results demonstrated that most of IKHs were lack of general handling knowledge about food safety. About knowledge, there were 21.5% of IKHs without knowledge of egg selection; 60% of IKH without knowledge of cross-contamination during processing. About handling, there were 20.4% IKHs without awareness of product label data; 65% of IKHs without handling behavior of washing vegetable under running water tap; 16.0% of IKHs used the same chopping board without any separation between raw and cooked products.