Parası kalmadığı için otobüse binemiyordur ailesi porno izle ona daha yeni para gönderdiği için tekrar porno istemeye utanınca mecburen otostop çekmek için youporn çantasını alarak yol kenarına gelir etekli porno liseli türk kız yol kenarında dururken yanına yaklaşan porno kibar bir gencin onu gideceği yere kadar bırakmak porno izle istemesine çok mutlu olur arabaya bindiklerinde gideceği yer ile porno arabayı kullanan adamın gittiği yer arasında çok mesafe sex izle farkı olduğunu anlayan türk kız bu yaptığı porno indir iyilik karşısında arabada ona memelerini açar porno sapıklaşan adam yol kenarındaki hotelde durarak porno izle üniversiteli otostop çeken türk kızına odada sakso çektirip sikerSome Characteristics of the Bacteriocin and Cold Shock Protein of the Strain Lactobacillus Plantarum Ul485 Isolated from Chao of Hue Province in Vietnam| Abstract
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Abstract

Some Characteristics of the Bacteriocin and Cold Shock Protein of the Strain Lactobacillus Plantarum Ul485 Isolated from Chao of Hue Province in Vietnam

Author(s): Uyen Quynh Nguyen, Anh Hoang Thi Dong and Le My Thi Nguyen

Lactic Acid Bacteria (LAB) and bacteriocins have been applied in many fields in our life. Among LAB, Lactobacillus plantarum plays some essential roles such as starter cultures or probiotics. In this study, bacteriocin synthesized by the strain L. plantarum UL485 isolated from the fermented “Chao” of Hue province in Vietnam was partly characterized. In addition, cold shock protein (Csp) in the strain and the survival of the bacteria with temperature downshift after freezing were primarily studied. In particular, the effects of pH and temperature on the bacteriocin, containing in the culture supernatant of the strain were evaluated. The bacteriocin was also partly purified via cation exchange chromatography. After cloning, the deduced amino acids of the cold shock protein in the strain were identified. In addition, after freezing, the survival percentage of the bacteria with temperature downshift from 30°C to 8°C at the growing log phase increased in comparison with that of bacteria without temperature downshift.


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