Impact of Diflubenzuron on mortality level, biochemical parameters of embryonic (in ovo) and neo-hatched chicks of Gallus domesticus

Author(s): Rouabhi Rachid, Djebar-Berrebbah Houria, Djebar Mohammed-Réda

The struggle against the agricultural devastating is increased in few last years and many products are created to kill the harmful insects and bug, without controlling their effect on ecosystem and non-target animals. In present work, we try to investigate the effects of the Diflubenzuron (DFB), which is an Insect growth Regulator (IGR) on biochemical and embryonic development of hen’s eggs as a non-target for this pesticide. The pesticide is tested in ovo by injection in the egg air cell of three concentrations in strictly controlled conditions, to show the impact on some biochemical parameters. The treatment with 1, 10 and 20µg/egg of the pesticide induced some blood parameters perturbations, especially in 10 and 20µg according to the controls. The 20µg concentration induces the blood serum proteins decreasing, increasing of triglycerides, decreasing of cholesterol and increasing of Alanine amino transferase (GPT) and GOT. This concentration induced a great level of embryonicmortality. The 01 and 10µg concentrations perturb (increase/decrease these parameters).

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