Genetic Polymorphism of Luteinizing Hormone Receptor Gene in Relation to Fertility of Egyptian Buffalo

Author(s): ASA Sosa, Karima Gh M Mahmoud, MMM Kandiel, HAA Eldebaky, MF Nawito and MEA Abou E l-Roos

The aim of this study was to detect the genetic polymorphism of luteinizing hormone receptor (LHR) gene and its association with fertility in Egyptian buffalo using PCR-RFLP and sequencing analysis. A total number of 301 comprised of 100 fertile and 201 non-fertile buffalo were used. Gynecological examinations (aided by ultrasonography) were carried out twice for two successive weeks. The genomic DNA was extracted from females and the PCR product was assessed. The percentages of inactive ovary and endometritis (grad, I) were the most common reproductive problems. The mean uterine horn diameter was largest during follicular phase followed by luteal phase and inactive ovary (2.67 ± 0.11, 2.28 ± 0.09 and 2.18 ± 0.11 cm, respectively). The PCR product of the primer specific for LHR gene gave the specific band at size 303 bp.The amplified fragments remained undigested by HhaI restriction enzymes and all tested buffalo were genotyped as TT. DNA sequence of 259 bp out of the amplified 303 bp was determined. The sequence alignment of 259 bp of Egyptian buffalo LHR with published sequence (accession number: XM_006078413.1 and JQ885687.1, Bubalus bubalis) showed that the 259 bp segment possess identities at 100%. In conclusion, monomorphic pattern for LHR gene is considered a unique feature that may be related to the characteristic species in buffalo. Therefore, the polymorphisms and interaction with the fertility trait should be the subject for further research with a bigger number of animals.

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