Abstract

Extraction of peroxidase from various plant sources and its biodegradation studies on phenolic compounds

Author(s): Senthilkumar Rathnamsamy, Raveesh Singh, Rufus Auxilia, B.N.Vedhahari

The enzymatic approach for bio remediation process has gained attraction because of its significant action on pollutants and high catalytic action compared to that of the chemical catalyst. This study focuses on the action of peroxidase enzyme on phenolic compounds present in industrial wastes. Crude Peroxidase enzyme was extracted from vegetable sourcesradish, tomato, turnip and cabbage. The enzyme was found to be stable at an optimumtemperature range of 55-65° C and at an optimum pH of 6-6.5. Using Ammonium sulphate precipitation, the crude extract was purified and maximum yield was obtained when the salt concentration was 80%. Aqueous Two phase extraction was also carried out and peroxidise was separated from top phase. Gel permeation chromatography was also performed and it showed up to 9.43 fold purificationwithmaximumspecific activity of 10.94U/mg. The effect of inhibitor, Sodiumazide, on the enzyme was studied and it clearly indicated that the activity of the enzyme decreased with increase in concentration of the inhibitor. Phenol degradation by the enzyme peroxidase showed that increase in the degradation of phenol was observed at high concentration of enzyme. Maximum degradation was obtained by the enzyme from turnip. Thus peroxidase can be used for phenol degradation in near future.