Abstract

Effects of alpha-particle irradiation on cell injury and the expressions of insulin sensitive-related genes in 3T3-L1 preadipocyte

Author(s): Shao-wei Sun, Yong-zhen Gong, Qin-hui Tuo, Xiao-ying Zhang, Chao ke- Tang, Duan-fang Liao

Exposure of cells to densely ionizing radiation can initiate a series of molecular and cellular events, which result in the gain and loss of functions in critical regulatory genes and cellular damage. In the present study, alpha-particle radiation-induced insulin resistance and toxic injury to 3T3- L1 preadipocyte were observed, and the mechanismunderlying the action was investigated. 3T3-L1 preadipocytes were irradiated by different doses of alpha-particle at 0Gy , 0.3Gy , 0.6Gy , 1.0Gy , 1.5Gy , 2.0Gy , and 4.0Gy . Cloning efficiencywas investigated to evaluate the cellular toxic effect of alpha-particle irradiation on cellular viability. Cytokinesis-block micronucleus technique ( CB-MNT ) and single-cell comet assay COMET assay ÿ were used to observe alpha-particle irradiation-induced DNA damage. Expression of caveolin-1, IRS-1 (insulin receptor substrate-1 ) and GLUT-4 ( glucose transporter-4 ) were analyzed by western blot and Reverse-transcription PCR (RT-PCR).Alpha-particle irradiation inhibited cloning efficiency, and increased micronucleus cells and the DNAdamage in 3T3-L cells in a dose-dependent manner. Alpha-particle irradiation decreased the mRNAexpression of GLUT-4 and IRS-1, and inhibited the protein expression of caveolin-1. The present results indicate that alphaparticle irradiation induced toxic injury and insulin resistance in 3T3-L1 preadipocyte, which might be associated with inhibition of GLUT-4 and IRS-1 expression by down-regulating caveolin-1.


Share this       

Share this Page.

Table of Contents

Scimago Journal Rank

Flyer
izmir escort izmir escort bursa escort antalya escort izmir escort porno porno izle türk porno eskişehir escort bartın escort burdur escort izmir escort bursa escort porno indir izle escort izmir