All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Diversity of nitrite oxidizers in greenwater system of coastal aquaculture by metagenomic approach targeting NxrB genes

Author(s): V.Kathiravan, K.K.Krishnani, I.S.Azad, M.Kailasam, A.G.Ponniah

Greenwater culture system is based on the integrated culture of economically important fish in pens inside shrimp culture ponds. However, the mechanisms on how the system works to bioremediate chemical and microbial pollutants are still unclear. Nitrite oxidizing bacteria (NOB) are an essential functional group for oxidation of nitrite into less toxic nitrate. The gene NxrB is unique to NOB, which encodes nitrite oxidoreductase enzyme that helps in oxidation of nitrite into nitrate. In the present study, the diversity of NOB was analyzed using the nxrB gene as a functional marker for screening constructed metagenomic library. The clones obtained had less similarity with NxrB of Nitrobacter sp. PJN1 and N. hamburgensis. However, the phylogenetic analysis showed that the clones obtained exhibit similarity with the sequences of nitrate reductase (nar) from Nocardioides sp. and Mycobacterium sp. of Acitinobacteria group. These results indicate that NOB population in a greenwater system of coastal aquaculture is much more diverse, and thus could be useful for planning a bioremediation strategy for nitrogenous metabolites and also for understanding of nitrogen fluxes generated through nitrite oxidation in coastal shrimp aquaculture.


Share this       
Awards Nomination

Table of Contents

Google Scholar citation report
Citations : 875

BioTechnology: An Indian Journal received 875 citations as per Google Scholar report

Indexed In

  • CASS
  • Google Scholar
  • Open J Gate
  • China National Knowledge Infrastructure (CNKI)
  • CiteFactor
  • Cosmos IF
  • Directory of Research Journal Indexing (DRJI)
  • Secret Search Engine Labs
  • Euro Pub
  • ICMJE

View More

Flyer