Abstract

Development and Validation Method for the Determination of Sildenafil Citrate Tablets by using UV-Spectrophotometer in Pharmaceutical Formulation

Author(s): Alrasheed AW Mohammed, Salah Elnaiem Mohammed, Hanaa S Boshra and Muzamil Hassaballa Abdelmula

The objective of this paper is to describe the optimization, validation, and application of spectrophotometric technique for determination of Sildenafil Citrate in their pharmaceutical formulation (tablets). In this paper, a simple, rapid, accurate and sensitive spectrophotometric method has been developed and validated. The method is a direct spectrophotometric method based on thawed of sildenafil citrate in diluted hydrochloric acid. The maximum absorption wavelength for the determination of Sildenafil citrate was found to be 295 nm. Under the optimized condition, Beer’s law was obeyed in the concentration range from 5.0-40.0 μg/ml.


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