An attempt to reveal Ty3-gypsy retrotransposon-based S-SAP markers in chickpea

Author(s): Manoj K.Rajput, Kailash C.Upadhyaya

Retrotransposons are present in high copy number in plant genomes. They show a considerable degree of sequence heterogeneity and insertional polymorphism, both within and between species. The retrotransposon based genetic diversity analysis technique Sequence- SpecificAmplification Polymorphism(S-SAP) has recently been derived fromtheAmplified Fragment Length Polymorphism(AFLP) technique. It produces amplified fragments containing a retrotransposon LTR sequence at one end and a host restriction site at the other. This is the first attempt to develop gypsy retrotransposon-based S-SAP markers in chickpea. We tried to find possible S-SAP markers in 30 chickpea (Cicer arietinum L.) accessions widely grown around the world. Polymorphisms were detected in eight accessions, using PCR primers designed from the long terminal repeat (LTR) of the chickpea retrotransposon CARE-1 and a set of three selectiveMseI+3 primers. Fromthe 215 bands scored, 12were polymorphic.

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