Ovarian cancer is a potentially life-threatening malignancy that develops either in one or both the ovaries. Ovarian cancer is considered as a Âsilent killer.Â Among many tumor markers which have been identified in many tumors, Carbonic anhydrase IX&XII are unique member of the CAfamily and suggested to play a vital role in oncogenic processes. CA IX participates in the regulation of acid-base balance, cell proliferation, adhesion and malignant processes. In this study mRNA transcription was studied by reverse transcriptase PCR (RT-PCR).The products of PCRwere run on agarose gel electrophoresis which showed strong positive expression of CA IX & XII. The expression pattern of CA IX & XII suggested that they could also serve as a histopathological marker protein for hypoxia in malignant ovarian tumors.