Aflatoxins constitute secondary metabolites produced by some Aspergillus species. These chemicals can contaminate a number of crops bound to human consumption. Numerous strategies for the detoxification or inactivation of aflatoxins contaminated feed- stuffs have been used. Detoxification of aflatoxin B1 (AFB1) by irradiation is one of the economical and practical methods exists. In this review, the effect of different irradiation techniques including UV, gamma and microwave on aflatoxin structure is discussed. Also different quantitative and qualitative techniques for detection of aflatoxin including biological activity test, mass spectrometry, infrared spectrometry, HPLC analysis, thin-layer chromatography and UV spectral analysis are reviewd.