Abstract

The use of VEGF supplemented media for chondrogenic differentiation of adipose derived mesenchymal stem cells

Author(s): Jeanne Adiwinata Pawitan, Des Suryani, Jinia Lilianty,Reza Yuridian Purwoko, Isabella Kurnia Liem

Aim: to verify the potential ofVEGF supplementedDMEM-HGin inducing chondrogenic differentiation of adipose derived MSCs Experimental : Lipoaspirate derived cellswere cultured in 10%humanAB serumcontainingDMEM-HGthatwas supplemented byVEGF. The culture was observed daily, and when the cells attached on the culture vessel, the medium was changed, and further medium changes were done every 2-3 days. Cell growth was observed, and cell characteristics were identified morphologically with and without staining.After the primary culture (P0) was 70%confluent, the cells were detached and passaged. Passages were done until passage 5. For every passage, seeding number, the day of the first appearance of clones and micromasses and results of alcian blue staining for every passage were noted. Mean and standard deviation values of the day when the cells formed the first clone and when the first micromass appeared were computed. Result : VEGF supplemented human AB serum containing DMEM-HG yielded first clones on day 4.29 ± 2.05, and first micromass on day 7.69 ± 2.62, which was faster compared to the recently commercially available chondrocyte differentiation medium. Conclusion : theVEGF supplementedmediumcan be used as an alternative simple induction media for faster chondrocyte differentiation.