Selective isolation and screening of laccase producing Streptomyces

Author(s): Ronak Chhaya, H.A.Modi

A novel streptomycetes, designated strain was isolated from various soil types near Ahmedabad and Anand city, Gujarat, India. Pretreatment of wet heating for 4 h at 700C and phenol treatment of soil suspension were the most effective method for isolation. Strain produced a branched substrate myceliumand aerial hyphae that developed into short, compact, spiral spore chainswith grey rugose spores at the tips of the aerial hyphae. Bennet’s agar was found the most suitable medium for the recovery of rare Streptomyces. Their morphological characteristics were determined through electronmicroscopy. Streptomyces were cultivated on solid media containing indicator compounds used were tannic acid, Guaiacol, oanisidine and p- anisidine for Laccase producing Streptomyces also showing positive Bavendamm’s reaction. Results of morphological and biochemical characteristics and the 16S rRNA gene partial sequences indicated this strain belonged to the genus Streptomyces. The strain formed a monophyletic line in a phylogenetic tree of 16S rRNA gene sequences with other Streptomyces reference strains. Growth curve analysis by dry mass method was studied.. Growth condition was optimized using an agro waste such as rice bran and wheat bran by solid state fermentation. Enzyme assay was carried out usingABTS.

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