RAPD-PCR and SDS-PAGE analysis of Aeromonas hydrophila for defining molecular characterization

Author(s): Agniswar Sarkar, Mousumi Saha, Avijit Patra, Pranab Roy

Aeromonas hydrophila isolated from fish (Labeo rohita), pond water, river water, rawmeat of chicken andmutton and rawcowmilkwere characterized through RandomlyAmplified Polymorphic DNA(RAPD) analysis and SodiumDodecyl Sulphate- Polyacrylamide Gel Electrophoresis (SDS PAGE) of cellular proteins. PCRamplification of theDNAfromthe bacterial isolates using ten random primers (OPA-01 to 10) were used and found some distinct bands on agarose gel in gel documentation system. Absolute polymorphismof RAPD profile was studied with a unique pattern for each isolate, indicating its usefulness as an ideal tool formolecular characterization, which are not revealed by other methods like morphological, serological, biochemical characterization and cellular protein profiling. Cellular protein profile did not produced significant polymorphism as all the isolates revealed uniform pattern indicating its usefulness as a tool for species level identification.

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