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Quick separation and determination of 6 lignans in Schisandra Chinensis (Turcz.) Baill and Schisand rasphenanthera Rehd. Et Wils using capillary electrophoresis

Author(s): Xie Yunliang, Zhang Yingjie, Zhang Bo

To set up a method for quick determination of 6 lignans in Schisandra Chinensis using capillary electrophoresis. The paper discussed the influence of factors, such as buffer solution, additives, separation voltage, temperature and sample-loading conditions, on the separation and determination of lignans. The Schisandra chinensis sample solution can be analyzed under the optimal conditions of 10mmol/L NaH2PO4-Na2HPO4 buffer solution (35% acetonitrile, 37.5mmol/L SDS at pH 8) and separation voltage 28.0KV. The linear range is 25.5~1020, 25.75~1030, 12~480, 25.5~1020, 24.25~970 and 19.75~790 mg/L respectively; the detection limit is 0.5, 0.8, 1.0, 1.0, 1.0 and 1.5 mg/L. By comparing to the high performance liquid chromatography, the present method has remarkable advantages in the separation effect, column efficiency and analysis speed

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