Production of cell free-amylase by immobilized Bacillus subtilis (ATCC 6633) under the substrate stress

Author(s): Jagdish Singh, Vishal Verma

Living cells of Bacillus subtilis were entrapped in calcium alginate beads for the -amylase production. There was maximum production after 120 Hrs at pH 7 when 8108 cell/ml immobilized in 2% sodium alginate was inoculated in the production medium containing 5% starch and calcium chloride as inducer. Amylase extracted by acetone precipitation has specific activity 29.341 IU/mg of protein, optimumpH7 and temperature 60C, Km 333µgm/ml and Vmax 1.5IU/ml/minute.

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