Preparation of high-biomass chromium-enriched saccharomyces cerevisiae cr-y yeastAuthor(s): Min Zhang, Jianhong Yang, Jie Zhang, Xi Li , Wenjun Ding
To obtain high chromium-collected Saccharomyces Cerevisiae, the optimal conditions for a strain of Saccharomyces cerevisiae N-Y which could tolerate high concentration of chromium were investigated. The optimal conditions for the N-Y by single factor and orthogonal array design methods was as follows: 30 g/L gucrose, 5 g /L NaNO4, 20 g/L yeast extract, 28~40ô¨, pH4~6, rotation speed at 200 rpm or more than 200 rpm. Subsequently, the N-Y was cultured in the optimal media and conditions with 3200 μg/ml chromium acetate acid for different time and was transfered at least 30 passages. We got a strain of Saccharomyces cerevisiae named Cr-Y which could collect chromium as 500 μg per gram dried yeast. The chromium-riched could reduce the sugar of diabetes mellitus type I animal model produced by steptotozocin obviously. The chromium collected from the Cr-Y was in the yeast, while other reported resulted suggested that part of the chromium was out of the yeast. The results of the sugar-reducing effects of our Cr-Y had been reported in other journal. The content of chromium was detected by ICP-MS.