Abstract

Optimization of mannanase production by Bacillus sp. MG-33

Author(s): Meenakshi, Gursharan Singh, Gurinder Singh Hoondal

Various Bacillus species were screened for the production of mannanase in submerged fermentation. Bacillus sp. MG-33 was selected as the most potent strain for production of enzyme with high activity. Organisim producedmannanase (13Uml-1) in presence of 0.5%guar gumas a sole carbon source inminimalmedia. Enzyme productionwas increased by 3.3, 2.5 and 1.0 fold when 0.25 and 1% DL-Leucine, wheat bran and tryptone were added to the minimal media respectively.When increasing (0.1-1.0%) the guar gum concentration in media then as a result linearly enhanced the production of mannanase by Bacillus sp. MG-33.