All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Optimization of fermentation conditions for human collagen a1(iii) chain production by pichia pastoris GS115 using response surface methodology

Author(s): Lin B.Li, Dai D.Fan, Xiao X.Ma, Pei Ma, Jian J.Deng

The optimal fermentation conditions for recombinant human collagen a1 (III) chain (rhCOL3A1) production by Pichia pastoris GS115 in a shake-flask culture was studied using statistical experimental design and analysis. The response surface methodology (RSM) was effective in optimizing conditions using the limited number of experiments. The optimal fermentation conditions for maximum rhCOL3A1 yield, was determined on the basis of a three-level three-factor Box-Behnken design (BBD), obtained by RSM. The high correlation between the observed and predicted values indicated the validity of the model. A maximum rhCOL3A1 yield of 508.46 mg/L was obtained at 28.2 oC, initial pH 4.95 and 1.35%/24h of methanol, which was 18.9% higher than production of 427.63 mg/L before optimization.


Share this       

Table of Contents

Recommended Conferences

International Congress on Biotechnology

Tokyo, Japan

24th Global Congress on Biotechnology

Dubai, UAE
Flyer
izmir escort izmir escort bursa escort antalya escort izmir escort porno porno izle türk porno eskişehir escort bartın escort burdur escort havalandırma izmir escort bursa escort porno indir izle escort izmir