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Obstacles in the Development of Stereo Selective Enzymes for Organic Chemistry

Author(s): Mary Garcia*

Thermo stability, substrate range, and enantioselectivity are just a few of the catalytic parameters of enzymes that can be improved through directed evolution for use in synthetic organic chemistry and biotechnology. When using synthetic organic chemistry with biocatalysts, stereo selectivity is very crucial. This article focuses on recent advances in approach for stereo selective enzyme evolution in the laboratory using hydrolases and monooxygenases as the enzymes. ISM or iterative saturation mutagenesis, has been found to be an incredibly effective way to evolve enzymes with improved or reversed enantioselectivity, a wider range of substrates, and/or increased thermo stability.

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