Abstract

Liquid Chromatographic And Spectrophotometric Determination Of Triprolidine Hydrochloride, Acrivastine And Pseudoephedrine Hydrochloride In Pharmaceutical Preparations And Human Plasma

Author(s): Feyyaz Onur, Ismail Murat Palabiyik, Erdal Din�������§

The present work describes four new lc methods and four chemometric techniques in spectrophotometry for the analysis of pseudoephedrine hydrochloride (PSE) - triprolidin hydrochloride (TRP) and pseudoephedrine hydrochloride (PSE) – acrivastine (AC) combinations in pharmaceutical preparations and in human plasma. In LC methods, ACE C18 column with a mobile phase composed of methanol – phosphate buffer (pH:7) (80:20, v/v) was used for PSE – TRP combination in pharmaceutical preparations and human plasma, and for PSE - AC mixture, same column with a mobile phase composed of methanol – phosphate buffer (pH:7) (95:5, v/v) was used in pharmaceutical preparations and a mobile phase composed of 0.1 M NaClO4 (pH:3)- acetonitril (95:5, v/v) by gradient elution technique was used in human plasma. Detection was at 220 nm for both combinations. Four chemometric techniques; CLS, ILS, PCR and PLS-1 methods were used for the spectrophotometric analysis of pharmaceutical formulations. In these techniques, the concentration data matrix were prepared by using the synthetic mixtures containing these drugs in 0.1M HCl for PSE – TRP mixture and in 0.1 M NaOH for PSE – AC mixtures. In the techniques, absorbance data matrix were obtained by the measurement of absorbances between 225.0 – 300.0 nm at 16 wavelengths in CLS, PCR and PLS–1 methods and between 245.0 – 300.0 nm at 12 wavelengths in ILS method in the zero-order absorption spectra of PSE – TRP mixture and between 240.0 – 285.0 nm at 19 wavelengths in the zero-order absorption spectra of PSE – AC combination in CLS, ILS , PCR and PLS–1 methods. The spectrophotometric procedures do not require any separation step. All the methods proposed were validated by analysing synthetic mixtures containing title drugs and they were successfully applied to the pharmaceutical formulations, capsule and tablet, and to human plasma and, the results were compared statistically with each other.