Abstract

Isolation of solid waste microflora

Author(s): S.Nataraja, D.M.Chetan, M.Krishnappa

Udupi district is a town located near Mangalore, Karnataka state. Temperature ranges from30 to 35 degrees centigrade in daytime and is around 22 - 22°C ± 2°C during night. Humidity is normally high most of the time. The rainy season is fromApril to September.Solid waste samples weighing approximately 2 to 2.5 Kgs were randomly taken fromKarkala and Udupi districts and then transferred into the nylon bags and brought to the laboratory for further evaluation for the incidence of bacteria.The objective of the project includes the collection of the Solid waste sample, isolation of themicroflora and then the identification of thesemicoflora.Differentmethods were adopted for the isolation of the micro organism. Some of these methods are Blotter method, Pour plate method,andAgar syringe method for bacteria. Identification of bacteria was done based on their colony and biochemical characteristics. Identification of fungi is carried out based on their colony and morphological characteristics, spore formation and biochemical reactions.The bacteria were isolated and identified to the species level. BergeyÂ’s Manual of Determinative Bacteriology was used as reference for identification.