Abstract

Induction of callus tissue and regeneration of Harmala (Peganum harmala L.) influenced by growth regulators

Author(s): Abbas Abhari, Jamal Bakhshi

There is limited information about the in-vitro propagation of harmal. This experiment was conducted as factorial in a completely randomized design with three replications in two separate experiments with different hormonal treatments. In the first experiment, MS medium containing growth regulators with different concentrations of BAP (0 mg/L, 0.5 mg/L, 1 mg/L and 2 mg/L) in combination with NAA at concentrations (0 mg/L, 0.25 mg/L and 5.0 mg/L) and in the second experiment, BAP with concentrations (0 mg/L, 0.5 mg/L, 1 mg/L and 2 mg/L) in combination with 2,4-D with concentrations (0 mg/L, 25 mg/L and 0.5 mg/L). The results showed that the highest callus and regeneration for the harmal plant was the combination of BAP hormone (0.5 mg/L) with NAA (0.5 mg/L). The results of the second experiment showed that callus of explants was carried out in most of the BAP and 2,4-D hormonal treatments and the highest regeneration was observed in the treatment of 1 mg/L BAP with 0.5 mg/L was observed. High BAP concentration in the presence of NAA decreased callus formation and appeared to be better for NAA callus enhancement. The high concentration of BAP in the presence of NAA reduced callus formation and it can be said that NAA was better for increasing callus production.