In vitro regeneration of Ocimum minimum L. - The smallest basil plant

Author(s): Nand Kishor Sharma, Vandana, Mithilesh Kumar

The purpose of present studywas to develop a micropropagation protocol of the important medicinal plant, Ocimum minimum L. from nodal stem explant. Nodal stem explants were inoculated on basal MS medium supplementedwith different concentrations of 6-benzylaminopurine (BAP), kinetin (KN) and combination of BAP with indole-3-acetic acid (IAA) for plant regeneration and 2,4-Dwere also used for callus induction.Maximum numbers of shoot (9.75±0.53) with highest length of shoots (5.95±0.08cm) were observed on the MS medium containing 1.0 mg/l BAP after 30-35 days of culture. Regenerated shoots were separated and rooted on half strengthMS medium supplemented with 1.0 mg/l of IBA alone for 25-28 days.Well-developed rooted plantlets were transferred to specially made plastic cup containing a mixture of garden soil, farm yard manure and sand (1:1:1) in controlled environment for longer duration for achieving high survival percentage (70-80%) for this plant. Acclimatized plantlets were successfully grown in garden soil. Thus, first time a comprehensive micropropagation protocol was developed for Ocimum minimum L. and it can be used for commercial propagation and in genetic improvement studies.

Share this       

Share this Page.

Table of Contents

Scimago Journal Rank

SCImago Journal & Country Rank