Functional evaluation of ToxA promoter in Trichoderma reeseiRut-C30

Author(s): Ortiz E.Gastón, Albertó Edgardo, BlascoMartín

In order to expand the collection of promoters studied in Trichoderma reesei Rut-C30, a functional study of the ToxA promoter was carried out by analysis of GFP expression in T. reesei Rut-C30. For this purpose, the binary pCBCT expression vectorwas employed to transformT. reesei Rut- C30. The transformants obtained were evaluated bymeans of fluorescence microscopy, fluorometry, dot blot andWestern blot analysis. The lowlevels of cytoplasmic GFP protein in fungal hyphae suggest that ToxA promoter works as a weak constitutive promoter which can drive successfully the expression of heterologous proteins in T. reesei Rut-C30.

Share this       

Share this Page.

Table of Contents

Scimago Journal Rank

izmir escort izmir escort bursa escort antalya escort izmir escort porno porno izle türk porno eskişehir escort bartın escort burdur escort izmir escort bursa escort porno indir izle istanbul escort istanbul escort bayan escort izmir