All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Establishment of a RT-lamp method for the rabbit hemorrhagic disease virus detection

Author(s): Yang Zexiao, Zeng Hui, Wang Yin, Yao Xueping, Wang Kaiyu

To develop a new molecular biological method for the quick detection of rabbit hemorrhagic disease virus (RHDV), a set of 7 primerswere designed according to the conserved sequence of the capsid proteinVP60gene of RHDV published inGenBank, and the reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was established through preparation of target gene fragments, optimization of reaction conditions, sensitivity and specificity tests. Results showed the RT-LAMPmethod for the RHDV detection had a ladder-like pattern of amplication bands from about 213 bp incubation at 64ºC for 45min by using agarose gel electrophoresis,and with good sensitivity and specificity,the detection limit could reach about 5 copies of cloned viral genomic fragments, which was more sensitve than that of traditional RT-PCR, and no amplifications for gene fragment of European brown hare syndrome virus, Pasteurella multocida, E.coli and Salmonella fromrabbits detection by this RT-LAMP approach.


Share this       
Awards Nomination

Table of Contents

Google Scholar citation report
Citations : 875

BioTechnology: An Indian Journal received 875 citations as per Google Scholar report

Indexed In

  • CASS
  • Google Scholar
  • Open J Gate
  • China National Knowledge Infrastructure (CNKI)
  • CiteFactor
  • Cosmos IF
  • Directory of Research Journal Indexing (DRJI)
  • Secret Search Engine Labs
  • Euro Pub
  • ICMJE

View More

Flyer