Abstract

Enhanced production of commercially importantL-asparaginase by a newly isolated strain of Bacillus sppmlo5 fromthe gut of dragon fly

Author(s): C.K.Venil, N.Sangeetha Kamatshi, P.Velmurugan, P.Lakshmanaperumalsamy

Microbial secondary metabolites represent a large source of compounds endowed with indigenous structures and potent biological activities. An enteric bacteria of Dragon fly (Libellula luctosa) Bacillus spp ML05 showed significant L-Asparaginase activity. The maximum production of L-asparaginase was noticed at 370C, pH6.5 and atmannitol (0.2%), ammonium chloride (0.4 %) and dipotassium hydrogen phosphate (0.8 %) were ideal chemical sources for the enzyme.All selected factors showed impact on L-asparaginase enzyme production by thismicrobial strain. Significant improvement in enzyme production from2.6 IU/ml to 4.2 IU/mlwas noted under optimized environment.