All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

A modified DNPH derivatization method in RPLC for quantification and determination of dihydroxyacetone and formic acid in formose samples

Author(s): Zafar Iqbal

Dihydroxyacetone (DHA) and formic acid (HCOOH) in formose samples were analyzed using reversed-phase liquid chromatography (RPLC) utilizing amodified chromatographic and 2, 4-Dinitrophynylhydrazine (DNPH)- derivatization techniques. Especial evaporation technique was developed to remove excess of unconverted substance from the formose mixture to facilitate the reduction of excess of reagent used for derivatization. Usage of derivatization reagent (DNPH) was reduced by a factor of 60. The optimumconditions for separation of DHA-hydrazone were (5-90)%ACN at 1 ml/min. Sodiumborohydride (NaBH4) reduction of aldehydes, ketones and sugars helped to analyze HCOOH-hydrazone quantitatively in formose sample.


Share this       
Awards Nomination 20+ Million Readerbase

Table of Contents

Google Scholar citation report
Citations : 378

Analytical Chemistry: An Indian Journal received 378 citations as per Google Scholar report

Indexed In

  • CASS
  • Google Scholar
  • Open J Gate
  • China National Knowledge Infrastructure (CNKI)
  • CiteFactor
  • Cosmos IF
  • Electronic Journals Library
  • Directory of Research Journal Indexing (DRJI)
  • Secret Search Engine Labs
  • ICMJE

View More

Flyer