Aim: To compare the growth factor contents of the various PRP processing methods to get an insight on the difference in growth factor yield from the various processing methods. Experimental: PRP before and after expiry dates was collected from Indonesian Red Cross. PRP was processed by various methods to release the growth factors, and growth factor levels were measured. Growth factor levels between various kinds of processing were compared usingANOVAand those frombefore and after expiry date samples were compared using t test. Results: Comparison of thrombin activation, one, two, and three freeze thawcycle(s) of all samples revealed no significantdifference inPDGF-AB,EGF, and IGFlevels (p>0.05).However, there were significant differences in TGFâ and VEGF levels between thrombin activation and freeze thawcycles. Comparisons between samples of before and after expiry date revealed no significant differences in all growth factor levels. Conclusion: One, two, and three freeze thawcycle(s) yielded no significant difference in most growth factor levels.