Abstract

Use Of Oxidation Reactions For The Spectrophotometric Determination Of Acyclovir And Amantadine Hydrochloride In Their Dosage Forms

Author(s): Ibrahim A.Darwish, Alaa S.Khedr, Hassan F.Askal, Ramadan M.Mahmoud

Five direct spectrophotometric methods for determination of acyclovir and amantadine hydrochloride has been developed and validated. These methods were based on the oxidation of the drugs by different inorganic oxidants: ceric ammonium sulphate, potassium permanganate, ammonium metavanidate, chromium trioxide, and potassium dichromate. Different variables affecting the reaction conditions were carefully studied and optimized. Under the optimum conditions, linear relationships with good correlation coefficients (0.9982–0.9999) were found between the reading and the corresponding concentration of the drug in the ranges of 2–1700 and 1-1100 μg/ml for acyclovir and amantadine HCl, respectively. The limits of detection ranged from 1.14-157.00 and 0.83-73.20 μg/ml for acyclovir and amantadine HCl, respectively. The precision of the methods was satisfactory; the values of relative standard deviations did not exceed 2%. The proposed methods were successfully applied to the analysis of acyclovir and amantadine HCl in their dosage forms with good accuracy and precisions; the label claim percentages ranged from 99.9–100.4 ± 0.62–1.05%. The results obtained by the proposed spectrophotometric methods were comparable with those obtained by the official methods.