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Abstract

Spectrophotometric methods for determination of Amprolium hydrochloride and Ethopabate binary mixture

Author(s): Lobna A.Hussein, Nancy M.Hanna, Mahmoud M.Abbas

Five simple, specific, accurate and precise UV- spectrophotometric methods are adopted for the simultaneous determination of Amprolium hydrochloride (AMP) and Ethopabate (ETH), a binarymixture with overlapping spectra without preliminary separation. The first method is first derivative of the ratio spectra (1DD) for determination ofAMP and ETH at 234.7 nm and 306.8 nmrespectively. The second method is the mean centering of the ratio spectra for determination of AMP and ETH at 238.8 nm and 313 nm respectively. The third method is based on dual wavelength selection for determination ofAMP and ETH at 235.3 nm&308 nmand 244 nm&268.4 nm respectively. The fourth method is ratio difference method for determination ofAMP and ETH at 239 nm&310 nmand 239 nm&313 nmrespectively. The fifth one is area under the curve (AUC) method where the areas between 235.6-243 nmand 268.3-275 nmare selected for determination of AMP and ETH simultaneously. These methods are tested by analyzing synthetic mixtures of the two drugs and they are applied to their pharmaceutical veterinary preparation. Methods are validated according to the ICH guidelines and accuracy, precision and repeatability are found to be within the acceptable limit.

Highlights •

These methods are sensitive and resolve binarymixture with overlapping spectra. • The good recovery and accuracymake themapplicable in QC laboratories • The proposed methods can be used in QC without the difficulties of HPLC • The proposed methods were validated according to ICH guidelines. • The proposed methods have been applied successfully on the veterinary formulation


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Analytical Chemistry: An Indian Journal received 378 citations as per Google Scholar report

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