Abstract

Spectrophotometric method for determination of midodrine hydrochloride using ninhydrin in pharmaceutical formulations

Author(s): Alaa El-Gindy, Shehab Sallam, Randa A.Abdel-Salam

A simple and fast spectrophotometric method has been developed for the determination ofmidodrine (MD) hydrochloride in pure formand in pharmaceutical formulations. The method is based on the reaction of the primary amino group of the drug with ninhydrin in methanol producing a Ruhemann purple coloured complexwhich absorbsmaximally at 576.5 nm. The effects of variables such as reagent concentration, reaction time, reaction tempreture, and Ruhemann purple color stability period, were investigated to optimize the procedure. The proposed method has been applied successfully to the analysis of the bulk drug and its pharmaceutical tablets and drops. No interference was observed from common pharmaceutical adjuvants.