Abstract
Simultaneous estimation of Ethyl Alfa Bromisovalerianat and Phenobarbitone in pharmaceutical formulation using HPLC.
Author(s): Safeena Sheikh, Patni Showkat, Anwar Daud, Suhail AsgharA simple, sensitive and isocratic reverse phase high performance liquid chromatographic (RPHPLC) method has been developed for the simultaneous determination of Phenobarbitone and Ethyl Alfa Bromisovalerianat in a formulation. HPLC analysis was carried out using reverse phase isocratic elution with a C18 column and a mobile phase of Acetonitrile: Water in the ratio of 65:35, v/v. Detection of the analytes were achieved using UV detector at 203 nm. The retention times of Phenobarbitone and Ethyl Alfa Bromisovalerianat were 3.90 and 9.8 minutes respectively Linearity of the method was found to be in the concentration range of 24- 144 ìgml-1 for Phenobarbitone and 20-120 ìgml-1 for Ethyl Alfa Bromisovalerianat. The correlation coefficient value was greater than 0.9999 for both the analytes. The method is suitable for the estimation of both the components simultaneously in pharmaceutical formulations.
