Immobilization of S. flexneri on low cost biopolymer based material for carbon dioxide sequestration

Author(s): RafatAnjum, Chandan Prabhu, Nitin Labhsetwar, Sadhana Rayalu

Bacteria was isolated, purified and screened for immobilization onmaterials. The screened isolate was identified as Shigella flexneri. The whole cell of Shigella flexneriwas immobilized on biopolymer (chitosan-clay and sodium alginate) based material. Optimal immobilization and esterase activity was determined for immobilized cells. Optimal immobilization timewas 6hwith shaking speed of 160 rpmand 200 rpmfor chitosan-clay beads and sodium alginate beads respectively. Immobilization of Shigella flexneri on sodium alginate beads is 25% higher than the chitosan-clay beads and may be attributed to increased number of reactive and functional group in sodium alginate beads compared to chitosan-clay beads. Based on p-NPA assay maximumesterase activitieswas observed to be 135U/ml for sodiumalginate beadswith specific activity of 985.44U/mg and 7.93 U/ml hydratase activity with specific activity of 21.90 U/mg. Similarly for chitosan clay, 127 U/ml esterase activities with specific activity of 520.81 U/mg and 7.21 U/ml hydratase activitywith specific activity 20.89U/mg. Sodiumalginate appears to better immobilizingmaterial than chitosan-clay.

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