Immobilization alludes to the way toward holding a joint or bone set up with a support, cast, or support. This is done to keep a harmed territory from moving while it recuperates. Immobilization is a procedure explicitly intended to limit the opportunity of development of a catalyst. Numerous procedures have been utilized already for protein immobilization, as entanglement, adsorption, covalent authoritative, embodiment, and cross connecting. The primary motivation behind supporting is to forestall development in the joints and bones above and underneath the break site. This is to keep bone edges from moving and making extra wounds by cutting tissue, muscle, vessels or nerves, conceivably transforming shut cracks into open breaks and making open injuries.   Plasma or forceful synthetic substances make responsive gatherings on PDMS that are exceptionally flimsy because of low glass progress temperature of - 120°C, however take into account further covalent immobilization of proteins. Shown this methodology by covalently immobilizing RGD peptides onto PDMS, which brought about satisfactory connection of A427 cells (a colon malignant growth cell line). Feinberg utilized this way to deal with immobilize fibronectin in a microprinted speck exhibit onto PDMS that bolstered an endothelial layer of an uncommonly high thickness for a culture time of as long as 18 days.