HPTLCmethod for simultaneous quantification of two biologically active compounds in the bark powder of Artocarpus lakoocha Roxb. Plant

Author(s): Nilan Rane, Vikas Vaidya, Maharudra Kekare, Willy Shah, Parikshit Champanerkar

Artocarpus lakoocha Roxb. belongs to family ofMoraceae. It is commonly called asMonkey jack. Artocarpus lakoocha Roxb. is a perennial tree found on west coast fromKokan southwards to Kerala and Tamil Nadu. In present study, a HPTLC method has been developed and validated for simultaneous determination of â-sitosterol and Lupeol in the bark powder of Artocarpus lakoocha Roxb. plant. Chromatographywas performed on silica gel 60 F254 HPTLCplate, with toluene:methanol, 8:1 (v/v), asmobile phase. After development, plates were treated with Lieberman-Burchard Reagent, detection and quantification were performed by densitometry at 366 nm in fluorescencemode. The method was validated in terms of its linearity, limits of detection and quantification and precision following standard protocols. â- Sitosterol and Lupeol was found to be linear in the range of 10 µg mL-1 – 40µg mL-1 for both. Average content of â- Sitosterol and Lupeol in Bark powder of Artocarpus lakoocha Roxb. was found to be 0.106 mg and 0.242 mg respectively. The method was found to be simple, faster, precise, sensitive, and accurate and can be used for routine quality control of herbal raw materials and for the quantification of these compounds in plant materials.

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