Abstract
High performance liquid chromatographic and ultra violet spectroscopic determination ofmesalamine in pharmaceutical formulations
Author(s): Sunil Majji, K.Srinivasa Rao, M.E.B.RaoAsimple, reliable and reproducible reverse phase HPLC and UV-spectroscopic methods were developed for the determination of mesalamine present in pharmaceutical dosage forms. The HPLC analysis was performed usingHypersilODSC18 (0.46cm25cm,5mm) columnfromShimadzu in isocratic mode, with mobile phase methanol: 10mMtetra butyl ammonium hydrogen sulphate (50:50%v/v) at a flow rate of 1ml/min with UV detection at 210 nm. In this HPLC method, zonisamide is used as internal standard. The retention timeswere 2.267min and 3.833min formesalamine and zonisamide respectively. UV-spectroscopic analysis was performed at 298 nm. Extraction of mesalamine from tablet was carried out using phosphate buffer (pH- 3.6). The linearity range was found to be 1-50 µg/ml for both HPLC and UV- methods. The results, obtained by the two methods in pharmaceutical preparation were compared. There were no significant difference between the mean values and the precision. These validated methods are suitable for the determination of mesalamine in pharmaceutical tablet formulations.
