Evaluation of content of â-sitosterol from stem bark and flowers of Salmalia malabarica collected from different regions

Author(s): Sunita Shailajan, Manasi Yeragi

A simple, rapid, selective and quantitative HPTLC method has been developed for the quantitation of â-sitosterol from Salmalia malabarica stem bark, flower collected fromdifferent regions and its formulations. Themethod was carried out using themethanolic extract of stembark, flower and formulation of Salmalia malabarica and was chromatographed on silica gel 60 F254 plates with Toluene- EthylAcetate-Methanol 7.0+1.0+0.5 (v/v/v) as mobile phase. Detection and Quantitation were performed by densitrometric scanning at ë= 366 nm using flourescence lamp. The plate was derivatised using 10%Methanolic sulphuric acid reagent followed by heating at 110°C for 10 minutes. The accuracy of the method was checked by conducting various validation parameters according to ICH guidelines. This method quantitates bioactive marker â-sitosterol as well as discriminates Salmalia malabarica stem bark from flowers which can be used as a quality control method to know the botanical identity of the plant raw material individually and in polyherbal formulation.

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