Development and validation of a RP-HPLC method for simultaneous determination of ritonavir and lopinavir in combined dosageform.

Author(s): Raja Abhilash Punagoti, Venkateshwar Rao Jupally

A reverse phase HPLC method was developed and validated for the simultaneous determination of two antiviral drugs viz. lopinavir and ritonavir. Chromatographywas carried out onC18 Column, Inertsil (150 x 4.6mm, 5 ì) withmobile phase of Buffer:Methanol:Acetonitrile (30:60:10v/v/v) pH 6.5, at a flowrate of 0.6ml/min and detection wasmade at 226 nm. The different analytical performance parameters such as linearity, precision, accuracy, specificity, limit of detection (LOD), limit of quantification (LOQ) and robustness were determined according to ICH guidelines. The linearity for each analyte in the desired concentration range was good (r2 >0.9). The recovery of themethod was 99.86%and 100.16%for ritonavir and lopinavir respectively. Hence the proposed method was sensitive, precise and accurate and it can be successfully apply to estimate the amount of ritonavir and lopinavir in the formulations by easily available low cost materials.

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