Determination of L – arginine in human urine and blood serum via CFIA / merging zones technique with spectrophotometric detection

Author(s): Bushra B.Qassim

A new FIA method is described for the determination of L – arginine in blood and urine samples. The method is characterized by sensitivity, simplicity and fast, it is based on formation a red color product between Larginine and á-naphthol in the presence of sodium hypobromite, as oxidizing agent which oxidized the reagent of á- naphthol, the oxidizing product condenses with L – arginine, the colored product measured at ëmax 501 nm. Chemical and physical parameters have been investigated, the linear dynamic range of L-arginine was 3-1400µg.ml-1 while correlation coefficient (r) was 0.9979, percentage linearity (r2%) C.O.D. was 99.58 %. Limit of detection (1µg.ml-1) for the lowest concentration in the linear dynamic range of the calibration graph with RSD% (n=10)  2%for concentration 60 and 800µg. ml-1. The method has been successfully applied for the estimation of L- Arginine in blood & urine samples and the FIA procedure is able to estimate these samples with a through put of 52 sample/ h. A comparison was made between the official method with the developed method via the paired t-test and F- test. It’s indicate that there were no worthy differences between either methods. therefore the new developed method of FIA can be adopted as a substitute method for determination of L-arginine.

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