Abstract
Derivative spectrophotometric method for determination of diacerhein and aceclofenac in pharmaceutical formulations
Author(s): Kirti S.Topagi, Mohit G.Dewani, Ashwini R.Madgulkar, Mrinalini C.DamleA simple, accurate and precise spectrophotometric method was developed for simultaneous estimation of Diacerhein and Aceclofenac in tablets by using first order derivative zero-crossing method. Aceclofenac showed a zero crossing point at 275.8 nm while Diacerhein showed a zero crossing point at 293.4 nm. The dA/dë was measured at 293.4 nm for Aceclofenac and 275.8 nm for Diacerhein and calibration curves were plotted as dA/dë versus concentration, respectively. The method was found to be linear in the range of 10-50 ìg/mL for both the drugs. The limit of detection was found to be 0.48 and 0.53 ìg/mL for Aceclofenac and Diacerhein respectively. The limit of quantitationwas 1.46 and 1.6 ìg/mL forAceclofenac and Diacerhein respectively. The method was successfully applied for simultaneous determination ofAceclofenac and Diacerhein in formulation.
