Batch and flow-injection spectrophotometric determination of vancomycin hydrochloride in pharmaceutical preparations using diazotized procaine penicillin

Author(s): Sadeemsubhi abed, OmarT.Hussein

New, simple and sensitive batch and Flow-injection spectrophotometric methods for the determination of Vancomycin Hydrochloride in pure form and in pharmaceutical preparations were proposed. These methods were based on diazotization and coupling reaction between Vancomycin Hydrochloride and diazotized Procaine penicillin in alkaline medium to form an intense yellow water-soluble dye that is stable and has a maximum absorption at 446nm. A graphs of absorbance versus concentration show that Beer’s law is obeyed over the concentration range of 0.8-40 and 5- 700 ìg.mL-1 of Vancomycin Hydrochloride with detection limits of 0.066 and 0.707 ìg.mL-1 of Vancomycin Hydrochloride for batch and FIAmethods, respectively. The FIA procedure sample throughput was 124h-1. All different chemical and physical experimental parameters affecting on the development and stability of the colored product were carefully studied and the proposed methods were successfully applied to the determination of Vancomycin Hydrochloride in pharmaceutical preparations.

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