Agnihotra is the basic home technique used since the ancient times in India as a religious ritual. It is said that theAgnihotra has medicinal implications and also known to control environmental pollution. Inspired of the above fact we conducted some experiments to check the antibacterial and antifungal activity of Agnihotra and we got an outstanding result. The Agnihotra was performed at times of sunrise and sunset exactly in a pyramid shaped copper vessel or Dharaka. Cow dung cake was used for firing. Unpolished and intact rice and pure ghee of native cow was added to the fire as Havissu. The experiment antimicrobial activity was done by Petri plate exposure method. The sterile nutrient agar(NA) and SabouraudÂÂs Dextrose agar(SDA) was exposed in a selected roomin tenminutes before performingAgnihotra. Another set of plates were exposed 10 min and 20 min after performingAgnihotra. The plateswere incubated at ambient temperature regimes and colony count was taken after incubation. According to the results obtained, it was concluded that Agnihotra was found to be very effective against Airspora. A 63% reduction in bacterial colony and 91% reduction in fungal colony count was obtained after performing Agnihotra.