Spectrophotometric and colorimetic methodology to detect and quantify hydrazide based chemotherapeutic drugs

Author(s): Ronald L.Bartzatt

Pharmaceuticals having the hydrazide functional group are an important division within anti-tuberculosis, anti-parasitic, anti-cancer, and anti-radiation chemotherapeutics. This work presents a colorimetric methodology to detect and quantify hydrazide based pharmaceuticals. Processes for rapid spot test, qualitative colorimetric assay, and spectrophotometric quantitative assay are presented. The reagent 2,6-dichloroquinone-4-chloroimine (Gibb’s reagent) is utilized to accomplish the various analytical objectives. The rapid spot test and qualitative colorimetric assay enables a detection of hydrazide drugs to a level of 0.0037 grams per milliliter. Quantitative spectrophotometric detection of hydrazide drugs is sensitive to a level of 128.2 micrograms permilliliter.Themolar absorptivity () is calculated to be 25.32 Liters/(molecm) at 970nm. Inorganic salts and organic compounds were found not to interfere with colorimetric detection of hydrazide drugs, and this includesNaBr,NaCl,MgSO4, 2-naphthol, benzoin, p-aminobenzoic acid various other compounds. Reagents necessary for this methodological approach are readily available. The colorimetric response of these tests is readily identified by visual inspection. These methods then are highly sensitive and provide a manner to determine this important type of drugs from environmental as well as biological origins.

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