Parası kalmadığı için otobüse binemiyordur ailesi porno izle ona daha yeni para gönderdiği için tekrar porno istemeye utanınca mecburen otostop çekmek için youporn çantasını alarak yol kenarına gelir etekli porno liseli türk kız yol kenarında dururken yanına yaklaşan porno kibar bir gencin onu gideceği yere kadar bırakmak porno izle istemesine çok mutlu olur arabaya bindiklerinde gideceği yer ile porno arabayı kullanan adamın gittiği yer arasında çok mesafe sex izle farkı olduğunu anlayan türk kız bu yaptığı porno indir iyilik karşısında arabada ona memelerini açar porno sapıklaşan adam yol kenarındaki hotelde durarak porno izle üniversiteli otostop çeken türk kızına odada sakso çektirip sikerComparative Studies on the Reaction Mechanism of Ceftazidime with Trypsin by Fluorescence Spectroscopy and Synchronous Fluorescence Spectroscopy| Abstract

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Abstract

Comparative Studies on the Reaction Mechanism of Ceftazidime with Trypsin by Fluorescence Spectroscopy and Synchronous Fluorescence Spectroscopy

Author(s): Baosheng Liu, Jinju Wang, Gang Bian, Zhiyun Li, Chundan Wang and Lihua Ma

The reaction mechanism of ceftazidime (CFD) with trypsin (TRP) was investigated using fluorescence spectroscopy and synchronous fluorescence spectroscopy at different temperatures (298, K 303 K and 310 K). The results showed that electrostatic force played a major role on the conjugation reaction between TRP and CFD, and the type of quenching was static quenching. The binding constants obtained using the above methods were of the same order of magnitude and very similar. Static electric forces played a key role in the interaction between CFD and TRP, and the number of binding sites in the interaction was close to 1. The donor-to-acceptor distance r<7 nm indicated that the static fluorescence quenching of TRP by CFD was also a non-radiation energy transfer process. The results of two methods were consistent that showed the synchronous fluorescence spectroscopy could be used to study the reaction mechanism between drug and protein, and was a useful supplement to the conventional fluorescence quenching method.


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