Wastewater generated from wastewater treatment plant contains odors some non pathogens, fecal Coliforms, fungus etc.,And chemicals such as phenols, nitrate, chloride etc. In addition to usual constituents such as urine and faeces. The present investigation was hence made in wastewater treatment plant situated in kallaraimedu of Kodaikanal. Presently the waste water is passing through an underground system to the wastewater plant. In this studyAnalyze the Physio-chemical parameters and biological parameters of septic water before and after treatment with horse and cow dung; and portable water. Isolate and identify the microbes present in horse and cow dung by using biochemical tests.