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The Release Property of Amoxicillin Nanoparticle and their Antibacterial Activity.

Author(s): Maysaa Ch AL-Mohammedawi, Rana A Mohsien, Mokhtar J Kadhim, Enas S Bahjat, Duna Q Al Azawi, and Samra A Qaraghuli

Early efforts have been made in an attempt to reduce the adverse side effect of multiple drug resistance organisms, and new classes of antimicrobial nanoparticles (NPs) and nanosized carriers for antibiotics delivery were developed. PURPOSE: This study was focused on the assessment of the physiochemical characterization, In vitro drug release, biofilm formation and antimicrobial properties of amoxicillin encapsulated within the Poly (ε-caprolactone) (PCL) nanoparticles. Methods: amoxicillin (AMX) nanoparticles were prepared using the emulsion solvent evaporation method with different concentrations of polycaprolactone (PCL) and Poly Vinyl Alcohol (PVA). These nanoparticles were subsequently characterized and evaluated for their antibacterial activity and biofilm inhibition activity using mean and standard error for Data analysis. Results: It was found that increased PCL concentration resulted in an increase in entrapment efficiency (EE%) to 83.3%. Meanwhile, an increase in the PVA concentration led to a decrease in the EE% and an increase in nanoparticle size. Enhancements in the percentage of practical yield to 80.2% as the polymer concentration rose. Fourier transform infrared spectra data for the MNPs, CS-coated AMX, and AMX-PCL-NPs nanoparticles were compared, which confirmed the PCL coating on the AMX and the AMX-PCL-NPs loaded nanoparticles. In addition, the antimicrobial activity of the nanoparticles e was determined using agar diffusion and growth inhibition assays against both gram-positive Staphylococcus aureus, gram-negative Pseudomonas aeruginosa and Proteus mirabilis bacteria. Furthermore, 10 µg/ml was the minimum inhibitory concentration of the AMX-PCL-NPs nanoparticle which inhibited biofilm formation in Staphylococcus aureus bacteria. Conclusion: Thus, this study presents a novel ß-lactam antibacterial-nanocarrier system that can reduce and inhibit bacterial growth showing it to be a promising tool for numerous medical applications

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