All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.


Synthesis of fibrous and non-fibrous mesoporous silica magnetic yolkâ??shell microspheres for immobilization of Lipase

Author(s): Zafar Ali

 The mesoporous yolk-shell nanoparticles (MYSNs), with a movable Fe3O4 core inside the hollow capsules, with two different morphologies (1)Fe3O4@SiO2@hollow@fibrous SiO2 (Yolk Shell-1) (2)Fe3O4@SiO2@hollow@mesoporousSiO2(Yolk Shell-2) were fabricated as carriers for Candida rugosa lipase (CRL) immobilization, through a template-assistant selectively etching method. Firstly the hydrophobic Fe3O4 nanoparticles were synthesized according to the solvothermal method. Then, SiO2 shell was coated on the Fe3O4 nanoparticle surface by a sol–gel reaction. Subsequently, the resorcinol farmaldehyde (RF) shell was covered on the surface of as-prepared Fe3O4@SiO2 composites and sandwiched Fe3O4@SiO2@RF composites were formed, which were finally provided with mesoporous fibrous (KCC) in the Yolk Shell-1 and mesoporous Silica in the Yolk Shell-2(non fibrous ). After selectively etching CTAB and the middle RF layer, the two kinds of yolk/shells composites were obtained. By utilization of their large and open mesopores (20-50nm) with good surface area (457.78-625.54 m2/g,) CRL was immobilized after amino functionalization using glutaraldehyde as a linker. The ICRL on Y.S-1, Y.S-2 showed good loaded (797-501 mg/g) and enzyme activity of (1503-837 U/g) respectively, thermal stability, resistance to pH, recycling and storage capacity were improved as compare to free lipase.


Share this       
Awards Nomination

Table of Contents