Parası kalmadığı için otobüse binemiyordur ailesi porno izle ona daha yeni para gönderdiği için tekrar porno istemeye utanınca mecburen otostop çekmek için youporn çantasını alarak yol kenarına gelir etekli porno liseli türk kız yol kenarında dururken yanına yaklaşan porno kibar bir gencin onu gideceği yere kadar bırakmak porno izle istemesine çok mutlu olur arabaya bindiklerinde gideceği yer ile porno arabayı kullanan adamın gittiği yer arasında çok mesafe sex izle farkı olduğunu anlayan türk kız bu yaptığı porno indir iyilik karşısında arabada ona memelerini açar porno sapıklaşan adam yol kenarındaki hotelde durarak porno izle üniversiteli otostop çeken türk kızına odada sakso çektirip sikerSynthesis of fibrous and non-fibrous mesoporous silica magnetic yolk???shell microspheres for immobilization of Lipase| Abstract

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Abstract

Synthesis of fibrous and non-fibrous mesoporous silica magnetic yolk???shell microspheres for immobilization of Lipase

Author(s): Zafar Ali

 The mesoporous yolk-shell nanoparticles (MYSNs), with a movable Fe3O4 core inside the hollow capsules, with two different morphologies (1)[email protected]@[email protected] SiO2 (Yolk Shell-1) (2)[email protected]@[email protected](Yolk Shell-2) were fabricated as carriers for Candida rugosa lipase (CRL) immobilization, through a template-assistant selectively etching method. Firstly the hydrophobic Fe3O4 nanoparticles were synthesized according to the solvothermal method. Then, SiO2 shell was coated on the Fe3O4 nanoparticle surface by a sol–gel reaction. Subsequently, the resorcinol farmaldehyde (RF) shell was covered on the surface of as-prepared [email protected] composites and sandwiched [email protected]@RF composites were formed, which were finally provided with mesoporous fibrous (KCC) in the Yolk Shell-1 and mesoporous Silica in the Yolk Shell-2(non fibrous ). After selectively etching CTAB and the middle RF layer, the two kinds of yolk/shells composites were obtained. By utilization of their large and open mesopores (20-50nm) with good surface area (457.78-625.54 m2/g,) CRL was immobilized after amino functionalization using glutaraldehyde as a linker. The ICRL on Y.S-1, Y.S-2 showed good loaded (797-501 mg/g) and enzyme activity of (1503-837 U/g) respectively, thermal stability, resistance to pH, recycling and storage capacity were improved as compare to free lipase.

 


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