Parası kalmadığı için otobüse binemiyordur ailesi porno izle ona daha yeni para gönderdiği için tekrar porno istemeye utanınca mecburen otostop çekmek için youporn çantasını alarak yol kenarına gelir etekli porno liseli türk kız yol kenarında dururken yanına yaklaşan porno kibar bir gencin onu gideceği yere kadar bırakmak porno izle istemesine çok mutlu olur arabaya bindiklerinde gideceği yer ile porno arabayı kullanan adamın gittiği yer arasında çok mesafe sex izle farkı olduğunu anlayan türk kız bu yaptığı porno indir iyilik karşısında arabada ona memelerini açar porno sapıklaşan adam yol kenarındaki hotelde durarak porno izle üniversiteli otostop çeken türk kızına odada sakso çektirip sikerSustainable Biodiesel Production from a Newly Isolated Micro-Algal Species Chlorococcum Sphacosum Using a Novel Lipase Immobilised Functionalized Nanobiocataylst| Abstract

44 7460 854 031

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Abstract

Sustainable Biodiesel Production from a Newly Isolated Micro-Algal Species Chlorococcum Sphacosum Using a Novel Lipase Immobilised Functionalized Nanobiocataylst

Author(s): Micheal Donatus S, Vijayalakshmi S and Ranjitha J

This research paper is mainly focused on the sustainable biodiesel production from a newly isolated micro-algal strain Chlorococcum sphacosum using a novel lipase immobilised functionalised nano-biocatalyst. In this reaction, the lipase was produced from Aspergillus niger (KP001169) a fungal strain isolated from oil contaminated soil sample collected nearby to the oil industry. The lipase was immobilized with magnetic nanoparticle using co-precipitation method and used as a nano-biocatalyst for the production of biodiesel production. The synthesized nano-biocatalyst enhanced the percentage yield of biodiesel. Using the lipase immobilised magnetic nanoparticles as a biocatalyst achieved maximum percentage yield 88% respectively. The catalyst can be reused more than five times in the biodiesel reaction without any treatment and activity changes and then after sixth cycle there is a loss in the activity of the synthesized nano-biocatalyst. Finally, the Fatty acid methyl esters (FAMEs) composition was characterized using GC-MS analytical technique.


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