Preparation Of Catalytic Materials By Trypsin Immobilisation On Carboxylic Textile And Carbon Matrixes Activated By Dicyclohexylcarbodiimide (DCC)

Author(s): N.Bouhaine, A.Djerourou, C.Innocent and P.Seta

New catalytic materials have been prepared by immobilisation of a protease, the trypsin, on a carboxylic cation exchange textile and carbon tube matrixes. In both cases the catalytic activity decreases in time like the soluble enzyme. Then, it takes a value nearly constant beyond 20 or 30 days and the materials keeps until 40% of its activity. In the case of the soluble enzyme this activity was losted continually during time. The Michaelis constants, Vm and Km of the immobilized enzyme were lower than that of the soluble enzyme due to the less accessibility of the substrate to the enzyme active sites.  2006 Trade Science Inc. - INDIA

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